| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,654,574 | 0 | A | G | 72.6% | 243.9 / 94.3 | 186 | N407N (AAT→AAC) | yifK | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (51/0); new base G (135/0); total (186/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 186 total aligned reads displayed.
CTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGC > W3110S.gb/3654519‑3654599 | ctacATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:445011/4‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTGCGTAGTTGGc > 1:1228971/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGtt > 1:786572/1‑58 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGtt > 1:358385/1‑58 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTgga > 1:655129/1‑60 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTgg > 1:1306537/1‑60 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTgg > 1:864917/1‑60 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTgg > 1:720659/1‑60 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:100472/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:517375/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:511828/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:505291/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:494571/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:489111/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:47345/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:401133/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:608353/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:346245/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:335968/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:317631/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:313308/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:304690/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:288193/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:284241/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:706312/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:978255/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:926833/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:922220/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:918637/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:801584/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:800201/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:742530/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:73927/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:539373/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:691321/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:688525/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:685750/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:652793/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:621881/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:370962/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:606274/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:559864/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:120879/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1382334/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1375546/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1325406/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1313257/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1291484/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1258747/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1216457/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1216448/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:252103/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1124032/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1109975/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1098596/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1097695/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1092290/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1050072/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1012974/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1434624/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:187336/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:170735/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:148921/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:145721/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:205361/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1444326/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:227505/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:282621/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1407703/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1414348/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTCGc > 1:1347995/1‑61 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGcc > 1:1276658/1‑62 (MQ=255)cTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGCAATGCCATTGTTACGTAGTTgg > 1:391259/1‑60 (MQ=255)cTTCATTAAAGTACATGCAGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAGTTGGc > 1:1338139/1‑61 (MQ=255) aTCAACACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:88101/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGcc > 1:1180819/1‑42 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAg > 1:933213/1‑60 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:891017/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:7288/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:853343/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:835626/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:899452/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:959150/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:809520/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:1057579/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:1025782/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:1092986/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:753371/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:731240/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:1396619/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:1147551/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:372969/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:668866/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:633113/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:420967/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:1313087/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:565907/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:446182/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:1365557/1‑61 (MQ=255) aTCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGc > 1:475130/1‑61 (MQ=255) | CTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCCATTGTTACGTAATTGGCCCACGGGAACAGGATTGAGC > W3110S.gb/3654519‑3654599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |