Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,843,932 | 0 | A | G | 72.4% | 224.9 / 89.2 | 174 | F169F (TTT→TTC) | rfaC | ADP‑heptose:LPS heptosyl transferase I |
Reads supporting (aligned to +/- strand): ref base A (48/0); new base G (126/0); total (174/0) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
Only 100 of 174 total aligned reads displayed.
TAGGCAGGTTCGTCAGAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACTTTT > W3110S.gb/3843916‑3843991 | tAGGCAGGTTCGTCTGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:693778/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCAt > 1:1413605/1‑36 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGccc > 1:8157/1‑44 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:569936/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:622851/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:556881/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:550359/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:547305/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:543998/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:533558/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:529306/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:505313/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:473060/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:463209/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:462402/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:417751/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:405772/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:398818/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:391685/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:373825/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:35073/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:329502/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:297817/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:824300/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:98223/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:968322/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:962243/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:937349/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:915628/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:896292/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:895000/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:847328/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:825872/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:595896/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:767118/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:765464/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:741890/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:716225/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:714002/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:67818/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:667640/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:644614/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1010066/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1407668/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1307884/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1245649/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1241485/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1211819/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1187985/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1138375/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1127924/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1107828/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1383140/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1060005/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1438025/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1036502/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1025210/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1002245/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:1469256/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:177742/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:189003/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:203247/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:203694/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:213888/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:249843/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:255353/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttg > 1:1336210/1‑59 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttg > 1:1321709/1‑59 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCGATAGCATAATCACCCTGggtttgtggtttgc > 1:626811/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAATGCTGTGCCATAGCATAATCGCCCTGggtttgtggtttgc > 1:993867/1‑60 (MQ=255) tAGGCAGGTTCGTCAGGAAAAGCTGTGCGATAGCATAATCGCCCTGggtttgtggtttgc > 1:632522/1‑60 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGc > 1:128973/1‑51 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:788531/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1218484/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:796081/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1328608/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:552363/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1130072/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1371329/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1377445/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1083673/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1074462/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1390810/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1450598/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1463447/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:429205/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:132006/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:622735/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:75195/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1236849/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:723743/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:597460/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:601768/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1268944/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:1287029/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:658393/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:648742/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:270061/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACtttt > 1:612983/1‑61 (MQ=255) gAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACttt > 1:1261549/1‑60 (MQ=255) | TAGGCAGGTTCGTCAGAAAATGCTGTGCGATAGCATAATCGCCCTGGGTTTGCGGTTTGCTATAGCCCAAACTTTT > W3110S.gb/3843916‑3843991 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |