Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,269,4580AC73.3% 93.6 / 41.3 75M29L (ATG→CTG) alralanine racemase 1, PLP‑binding, biosynthetic
Reads supporting (aligned to +/- strand):  ref base A (20/0);  new base C (0/55);  total (20/55)
Fisher's exact test for biased strand distribution p-value = 1.25e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCAC  >  W3110S.gb/4269405‑4269493
                                                     |                                   
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:972383/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:224540/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:198934/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:805797/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:819085/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:1452737/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:852162/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:1427061/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:1415404/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:859699/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:138217/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:1375757/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:1355127/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:1091553/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:907404/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:1096014/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:965420/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                             >  1:1145644/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTAGCg                             >  1:649776/1‑62 (MQ=255)
gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCACTGCCAGTAAAATGGTTGCg                             >  1:332954/1‑62 (MQ=255)
                                             ccAGTAAACTGGTTGTGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1312349/44‑1 (MQ=21)
                                             ccAGTAAACTGGTTGCTGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:180649/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTGAc  <  1:1319788/44‑1 (MQ=21)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:641434/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:389316/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:636812/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:529996/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:654863/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:539253/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:59202/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:629487/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:511814/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:656984/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:659446/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:732951/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:735528/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:779813/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:802460/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:822828/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:85893/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:888591/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:891557/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:892573/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:896826/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:915449/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:168058/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1078266/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1105297/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1162594/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1231788/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1254532/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:128066/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1303142/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1345776/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1407935/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1430121/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1433362/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1441503/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1457052/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1469844/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:50414/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:171972/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:215239/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:246408/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:269718/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:354313/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:1058339/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:390462/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:403657/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:411296/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:438663/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:470914/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:482498/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGTCAc  <  1:48729/44‑1 (MQ=25)
                                             ccAGTAAACTGGTTGCGGTGGTGAAAGCGAACGCCTACGGGCAc  <  1:249447/44‑1 (MQ=18)
                                                     |                                   
GCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCAC  >  W3110S.gb/4269405‑4269493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: