Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,333,6580CT73.3% 162.2 / 64.5 131T615I (ACT→ATT) yjdAconserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base C (35/0);  new base T (96/0);  total (131/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 131 total aligned reads displayed.

TGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAT  >  W3110S.gb/4333647‑4333708
           |                                                  
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGaaa   >  1:1119588/1‑61 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1187949/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1198676/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1205202/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:601528/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1278992/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1311596/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:216383/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1344457/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:619535/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:772817/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:19329/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:172682/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1388806/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:160930/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1467431/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:643839/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:347349/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1173643/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:345650/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:875406/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1069366/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:528512/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1177632/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1133794/1‑62 (MQ=255)
tgtgCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt  >  1:1107560/1‑62 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACt                       >  1:805280/1‑39 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGaaa          >  1:526616/1‑52 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:495932/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:430499/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:624674/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:453785/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:462337/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:480385/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:618281/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:581996/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:572908/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:792016/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:561281/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:861933/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:989275/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:966887/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:964545/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:951582/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:950009/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:941946/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:936327/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:926409/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:907608/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:865474/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:65077/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:816891/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:816496/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:815335/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:795766/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:264383/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:75638/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:728313/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:70893/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:663087/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1170620/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1363760/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1355830/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1341596/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1333698/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1252152/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:124030/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:123192/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1226704/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:119438/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:138034/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1170298/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1159009/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:114778/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1126567/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1119172/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1088014/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1056272/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1027214/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1007085/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:243626/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:392649/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:321122/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:319334/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:314502/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:311660/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:308822/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:276717/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1000926/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:253854/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:426053/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:2359/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:229826/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:21428/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1467492/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1451172/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1443001/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1430154/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:1417503/1‑53 (MQ=255)
  tgCGTGAGATTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt         >  1:138741/1‑53 (MQ=255)
           |                                                  
TGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAT  >  W3110S.gb/4333647‑4333708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑