| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,459,476 | 0 | C | T | 73.7% | 69.5 / 24.8 | 57 | L272L (TTG→TTA) | fbp | fructose‑1,6‑bisphosphatase I |
| Reads supporting (aligned to +/- strand): ref base C (0/15); new base T (42/0); total (42/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.53e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGT > W3110S.gb/4459426‑4459516 | ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:622323/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:200395/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:204744/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:217241/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:232655/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:238351/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:288086/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:288961/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:455011/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:54207/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:54357/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:580290/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:102723/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:641577/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:744072/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:779624/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:786425/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:828508/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:860369/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:8651/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:936838/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:960567/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:990662/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1237112/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1105029/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:107688/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1195927/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:104127/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1217562/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1082579/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1223844/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1100793/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1238323/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1036848/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1317184/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1008973/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1321371/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1327628/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:1465678/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:169013/1‑62 (MQ=255)ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGt > 1:725059/1‑60 (MQ=39)ttACCGCCCGCGTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGTAAACGTAGttt > 1:10949/1‑62 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:1066380/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:947049/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:952400/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:807320/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:199579/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:1092334/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:756530/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:733722/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:1146903/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:560990/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:1220806/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:325720/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:1283328/48‑1 (MQ=255) cATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:228824/48‑1 (MQ=255) aTACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGt < 1:638258/47‑1 (MQ=255) | TTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGT > W3110S.gb/4459426‑4459516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |