| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 75,154 | 0 | G | A | 74.6% | 243.5 / 82.2 | 177 | P109P (CCC→CCT) | tbpA | thiamin transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/45); new base A (132/0); total (132/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.83e-43 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 182 total aligned reads displayed.
ATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAT > W3110S.gb/75118‑75170 | aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCagg > 1:126438/1‑39 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCagg > 1:960417/1‑39 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:351692/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:60209/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:594320/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:563458/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:550178/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:526469/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:47140/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:433369/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:409787/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:387169/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:627282/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:314523/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:280926/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:275211/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:256051/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:244068/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:232868/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:21966/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:214653/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:776961/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:991033/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:966510/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:947922/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:909830/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:864446/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:859688/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:858815/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:826590/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:803595/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:615001/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:740680/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:723236/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:721400/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:702598/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:678401/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:672950/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:662745/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:643491/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1008127/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1190282/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1345219/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1291956/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1267416/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1262407/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1252178/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1241074/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1237333/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:123438/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:179520/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1146384/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1130844/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1119789/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1044650/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:10340/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1025618/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1018275/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:100132/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:205825/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1418776/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1418402/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1456683/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1384322/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1378201/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:137114/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:167677/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCAGGa > 1:1356197/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATTATTACAGCCGCCAGGa > 1:1066795/1‑40 (MQ=38)aTCAAACGGTACGAAAGTGTCATTACTCCAGCCGCCAGGa > 1:578563/1‑40 (MQ=255)aTCAAACGGTACGAAAGTGTCATAATTCCAGCCGCCAGGa > 1:343418/1‑40 (MQ=38) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:882909/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1135287/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1080836/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:844792/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:850259/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1449612/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1041086/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:159523/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:91779/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:930099/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:930653/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1022606/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1234006/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:502562/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:563840/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1274858/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:439589/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:631025/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:654921/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1407495/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:673998/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:788421/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1219099/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:723903/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:1349813/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:760138/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:770969/43‑1 (MQ=255) aCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:294130/43‑1 (MQ=255) cGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAt < 1:338074/42‑1 (MQ=255) | ATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCAT > W3110S.gb/75118‑75170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |