| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 699,390 | 0 | G | A | 71.4% | 204.3 / 111.0 | 199 | H70H (CAC→CAT) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base G (57/0); new base A (0/142); total (57/142) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 199 total aligned reads displayed.
CGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGT > W3110S.gb/699346‑699406 | cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:605643/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:168812/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:204587/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:240444/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:317803/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:345080/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:409085/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:440/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:477210/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:521687/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:537268/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:552908/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:1007319/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:660022/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:665776/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:676713/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:682913/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:714159/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:810475/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:869962/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:885208/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:912612/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:948572/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:993932/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:1004277/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:1305178/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:1171353/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:1222571/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:1141499/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:1045172/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTgttggt > 1:1026750/1‑61 (MQ=255)cgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCACTACGTGGGATTTTTgttgg > 1:1093793/1‑60 (MQ=255) tggtggTTGTAGATTTCACCGTTTACTGCTAGCACATga < 1:373891/39‑2 (MQ=21) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1122555/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:691986/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1136777/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:959023/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1143945/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:634944/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:628806/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1146700/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:593249/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:977140/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:587844/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:581670/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:559185/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:986067/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1188145/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:94201/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:712621/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1108780/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:720556/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:734589/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:748505/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:759771/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:777975/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:813709/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:826461/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1020679/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1038623/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:885844/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:894196/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:908577/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1430019/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:217021/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:208193/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:127099/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:185576/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1285123/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1467973/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1461104/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1455005/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:219957/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1418182/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1416286/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1388726/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1369913/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1349662/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1342693/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1332530/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1308106/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:336241/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1192979/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1204487/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:456796/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1206613/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:405152/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:392714/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:380815/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:350233/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1235879/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:54919/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:324737/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:1246407/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:262784/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:249015/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:126541/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:239539/39‑2 (MQ=25) tggtggTTGTAGATTTCACCGTTTACTGCCAGCACATga < 1:231841/39‑2 (MQ=25) ggtggtTGTAGATTTCACCGTTTACTTCCAGCACATga < 1:758080/38‑2 (MQ=16) | CGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGT > W3110S.gb/699346‑699406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |