Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	587,934	G→A	S748S (TCC→TCT)	nfrA ←	bacteriophage N4 receptor, outer membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	587,934	0	G	A	100.0%	234.4 / NA	85	S748S (TCC→TCT)	nfrA	bacteriophage N4 receptor, outer membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (85/0);  total (85/0)

AGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGC  >  W3110S.gb/587901‑587962                                  |                             agtggTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:115334/4‑62 (MQ=255) aGCTGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:753284/1‑62 (MQ=255) aGCGGTACTCATTGCGCCTGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1165238/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGaaa                  >  1:984689/1‑46 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGaa                   >  1:136144/1‑45 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGaa             >  1:958217/1‑51 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGa              >  1:461833/1‑50 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTc         >  1:1096595/1‑55 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgctgc  >  1:498170/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:556092/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:644166/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:615975/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:612957/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:604423/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:579323/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:576931/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:563957/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:557916/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:651816/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:552669/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:550548/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:535850/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:507850/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:505949/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:500279/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:478829/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:424719/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:836534/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:973959/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:946972/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:890483/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:882604/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:880216/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:873395/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:850606/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:837116/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:958553/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:818461/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:81714/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:794696/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:78052/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:995937/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:742488/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:725301/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:681902/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1287057/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:144306/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:44136/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1370095/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1330400/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1325645/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:131521/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1302963/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1299703/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1293169/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1434994/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1265374/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:125357/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1205470/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1182983/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1177687/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1135393/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1064696/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1057071/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:281319/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1030951/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:404984/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:365750/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:348068/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:337947/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:330399/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:319946/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1450229/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:279249/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:253220/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:230522/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:175035/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:174841/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:15455/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1466091/1‑62 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcgg   >  1:1208629/1‑61 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcgg   >  1:371534/1‑61 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcg    >  1:601056/1‑60 (MQ=255) aGCGGTACTCATTGCGCCGGAACGCAAGCCGATAGAAGAATCGAAACTGAACGTCCAgc     >  1:1319185/1‑59 (MQ=255) aGCGGTACTCATTGCGCCGGAACACAAGCCGATAGAAGAATCGAAACTGAACGTCCAgcggc  >  1:1129164/1‑62 (MQ=255)                                  |                             AGCGGTACTCATTGCGCCGGAACGCAAGCCGATGGAAGAATCGAAACTGAACGTCCAGCGGC  >  W3110S.gb/587901‑587962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑