| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 640,175 | G→A | L400L (CTG→CTA) | ahpF → | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 640,175 | 0 | G | A | 83.3% | 139.9 / 16.9 | 78 | L400L (CTG→CTA) | ahpF | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/13); new base A (0/65); total (0/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGT > W3110S.gb/640141‑640225 | aaaTTAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCTACattat < 1:1314130/61‑1 (MQ=255)aaaTGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:660770/61‑1 (MQ=255)aaaTGAAACCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:228058/61‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:470178/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:553550/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:545246/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:541419/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:510800/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:482910/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:474141/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:556302/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:46645/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:427877/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:406845/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:368310/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:330177/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:324222/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:29249/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1002629/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:572328/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:644796/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:704816/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:718690/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:737896/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:744231/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:786360/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:788368/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:793212/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:922140/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:928064/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:943545/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:949519/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:961783/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:971779/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1215864/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1367560/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1347708/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1327612/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:265939/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:125219/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1250169/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1250103/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:124560/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1220215/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1283212/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:118042/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:11760/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1168488/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1122715/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:109018/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1064018/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1010544/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1000327/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:260216/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1437273/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1440488/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1447529/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:249229/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:200701/58‑1 (MQ=255) tGAAAGCCGACCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:1459408/58‑1 (MQ=255) aCCAGGTTCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:315923/49‑1 (MQ=255) ggTTCTGCAGGACAATCTACGCAGGCTGAAAAACGTCGACattat < 1:588566/45‑1 (MQ=255) ttCTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:27562/43‑1 (MQ=255) cTGCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:301569/41‑1 (MQ=255) gCAGGACAAACTACGCAGCCTGAAAAACGTCGACattat < 1:704559/39‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:677440/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:444293/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:172335/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:4638/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:355908/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:21988/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:587781/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:1157321/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:58051/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:1122005/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:1433536/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:285528/60‑1 (MQ=255) ggACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGt < 1:1406640/60‑1 (MQ=255) | AAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATTCTGAATGCGCAAACCACGGAAGT > W3110S.gb/640141‑640225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |