Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	655,532	G→T	L287I (CTC→ATC)	dcuC ←	anaerobic C4‑dicarboxylate transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	655,532	0	G	T	100.0%	222.1 / NA	81	L287I (CTC→ATC)	dcuC	anaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (81/0);  total (81/0)

TCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGAGGAACTCCAGAATGGAGGCA  >  W3110S.gb/655492‑655551                                         |                    tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAATTCCAGAATGGAGGCa  >  1:1379127/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGa      >  1:371341/1‑56 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGa      >  1:152992/1‑56 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAgg    >  1:572975/1‑58 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGct  >  1:1315177/1‑59 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:573509/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:705451/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:701482/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:677983/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:645184/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:619837/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:617829/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:590256/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:481014/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:536744/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:524104/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:523852/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:52319/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:521909/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:516530/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:514278/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:398084/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:831103/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:975721/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:97461/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:959803/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:89964/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:892896/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:891793/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:891138/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:863967/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:84513/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:715471/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:823670/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:822802/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:800196/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:794888/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:784255/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:780203/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:777193/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:757547/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:728557/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1255270/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:471056/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1369022/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1363459/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1351249/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1334225/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1328984/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1324649/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1314523/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1280414/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1377359/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1247974/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:117557/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1172812/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1152784/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1141471/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1090769/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1034735/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1030852/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:277700/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:439450/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1016752/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:397563/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:395995/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:367721/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:350407/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:348449/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:333010/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1415479/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:249599/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:24270/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:16668/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1472517/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1446471/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1442463/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGATGAACTCCAGAATGGAGGCa  >  1:1418604/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGCATGAACTCCAGAATGGAGGCa  >  1:1262394/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCAGATGAACTCCAGAATGGAGGCa  >  1:148483/1‑60 (MQ=255) tCCAGACCAGAGAAAACTTTCTGGCTATTAAAGCTGCGGATGAACTCCAGAAt         >  1:90759/1‑53 (MQ=255)                                         |                    TCCAGACCAGAGAAAACTTTCTGGGTATTAAAGCTGCGGAGGAACTCCAGAATGGAGGCA  >  W3110S.gb/655492‑655551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑