| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 703,401 | C→T | A211A (GCG→GCA) | nagB ← | glucosamine‑6‑phosphate deaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 703,401 | 0 | C | T | 84.3% | 304.0 / 38.4 | 159 | A211A (GCG→GCA) | nagB | glucosamine‑6‑phosphate deaminase |
| Reads supporting (aligned to +/- strand): ref base C (25/0); new base T (0/134); total (25/134) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-29 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
TGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCCAGC > W3110S.gb/703369‑703455 | tGGTTCACGCAACCTTCAACTGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:478400/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGTCTACCCAGCACCAg < 1:569854/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:468581/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:595724/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:568707/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:548781/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:512514/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:500471/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:49831/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:479816/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:475546/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:607193/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:465763/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:45702/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:400150/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:380144/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:354701/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:343458/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:34061/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:334976/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:333558/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:822535/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:982787/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:932520/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:930560/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:918483/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:896326/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:870514/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:863976/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:855277/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:842827/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:828684/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:366274/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:792650/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:788948/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:711130/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:708980/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:704322/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:699057/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:69107/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:687579/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:670625/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1210318/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1418148/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1358470/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1350546/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1348677/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:331814/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1323978/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1316601/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1314903/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1314885/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1228371/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:133192/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1203444/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1186158/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1181976/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1141615/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1141277/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1080395/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1072020/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1045052/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1029940/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1006538/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1426684/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:145023/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1452796/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:306608/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:179599/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:265713/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:229107/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:213141/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:197246/62‑1 (MQ=255)tGGTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:196319/62‑1 (MQ=255) ggTTCACGCAACGTTCAACGGCGGCCTGCAGTGCCAGTGCTTTGTGACTACCCAGCTCCAg < 1:1168204/61‑1 (MQ=255) ggTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACGCAGCACCAg < 1:1397153/61‑1 (MQ=255) ggTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:623517/61‑1 (MQ=255) ggTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1346808/61‑1 (MQ=255) ggTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:814990/61‑1 (MQ=255) ggTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:351943/61‑1 (MQ=255) gTTCACGCAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:829492/60‑1 (MQ=255) cAACCTTCAACGGCGGCCTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:1268048/53‑1 (MQ=255) ggcggcCTGCAGTGCCAGTGCTTTCTGACTACCCAGCAcctg < 1:446070/42‑3 (MQ=37) ccTGCAGTGCCAGTGCTTTCTGACTACCCAGCACCAg < 1:100054/37‑1 (MQ=37) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:539036/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:419451/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:964162/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:953397/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:1018631/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:1044980/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:263624/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:194423/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:344310/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:1085568/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:42456/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:375766/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:755888/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:38776/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:389303/1‑62 (MQ=255) ccTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc > 1:1287735/1‑62 (MQ=255) | TGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCCAGC > W3110S.gb/703369‑703455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |