Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	810,050	C→T	R95R (CGC→CGT)	bioB →	biotin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	810,050	0	C	T	100.0%	179.5 / NA	65	R95R (CGC→CGT)	bioB	biotin synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/65);  total (0/65)

GCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGA  >  W3110S.gb/810033‑810072                  |                       gtggCGGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1337589/38‑1 (MQ=37) gcggcgGGATCGACGCGTTTCTGTATTGGCGCGGCGTGga  <  1:964140/40‑1 (MQ=37) gcggcgGGATCGACGCGTTTCTGTATGTGCGCGGCGTGga  <  1:389058/40‑1 (MQ=37) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTgta  <  1:1331175/40‑3 (MQ=37) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTTga  <  1:808496/40‑1 (MQ=37) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:495813/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:576737/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:47979/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:443171/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:437489/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:411791/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:39383/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:384386/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:378667/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:332152/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:239876/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1017749/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:557646/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:237394/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:579364/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:599973/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:603668/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:632336/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:685128/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:767178/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:774881/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:778055/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:810609/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:81386/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:915022/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:918747/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:969842/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1253171/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1029138/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1029592/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1083586/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1084876/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1088995/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1106151/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1112345/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1114380/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1115757/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1126163/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1127651/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1130397/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1172989/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1211173/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:237201/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1469436/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:218940/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:20363/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:190949/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:185260/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:17739/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:168609/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:125586/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1334961/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1320340/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1302775/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1301566/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:1282316/40‑1 (MQ=38) gcggcgGGATCGACGCGTTTCTGTATGGGCGCGGAGTGga  <  1:458579/40‑1 (MQ=37) gcggcgGGATAGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:635512/40‑1 (MQ=37)   ggCGGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:962021/38‑1 (MQ=37)   ggCGGGATCGACGCGTTTCTGTATGGGCGCGGCGTGga  <  1:295638/38‑1 (MQ=37)                  |                       GCGGCAGGATCGACGCGCTTCTGTATGGGCGCGGCGTGGA  >  W3110S.gb/810033‑810072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑