Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 820,127 T→C V137V (GTT→GTC)  moaE → molybdopterin synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb820,1270TC100.0% 338.9 / NA 118V137V (GTT→GTC) moaEmolybdopterin synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/118);  total (0/118)

Only 100 of 118 total aligned reads displayed.

GCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGG  >  W3110S.gb/820106‑820166
                     |                                       
ctcGGAAGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1407309/59‑1 (MQ=39)
 tcGGAAGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:836247/59‑1 (MQ=39)
     aaGGTGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1436595/56‑1 (MQ=38)
     aaGGCGACGGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:280770/56‑1 (MQ=38)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGGTCAGCAGGCGGCAAAACGCTgg  <  1:959008/56‑1 (MQ=38)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGGAAAACGCTgg  <  1:993555/56‑1 (MQ=255)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:693689/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:365146/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:65024/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:629511/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:626962/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:609122/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:590005/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:555468/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:521926/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:507384/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:500939/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:491719/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:465062/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:458098/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:457737/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:437223/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:416094/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:403712/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:394475/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:3696/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:36013/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:85373/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:961091/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:957471/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:950664/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:941447/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:924775/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:91338/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:905605/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:891226/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:881459/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:868343/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:863498/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:694097/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:835852/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:826890/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:774183/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:773975/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:721743/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:715225/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:714578/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:705067/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:70122/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:695648/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1210281/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1358809/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1349530/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1317931/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:131555/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1293268/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1290486/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1286459/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1270261/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1243311/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1241339/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1239581/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1232714/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1361064/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1204040/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1192545/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1191886/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1181771/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1153052/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1149744/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1113453/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:110802/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:10996/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1077438/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1044591/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:190041/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:305158/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:295365/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:289676/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:287470/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:287365/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:284562/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:274925/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:272390/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:258565/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:229097/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:204821/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1018473/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:177372/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1462924/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1445044/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1439584/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1429196/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1422157/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1376854/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:1362972/56‑1 (MQ=39)
     aaGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGACAAAACGCTgg  <  1:471308/56‑1 (MQ=255)
      aGGCGACCGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:55557/55‑1 (MQ=39)
          gACCGTTGGGTCGAAGCGCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:668495/51‑1 (MQ=37)
            ccGTTGGGTCGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTgg  <  1:162399/49‑1 (MQ=38)
                     |                                       
GCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGG  >  W3110S.gb/820106‑820166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑