Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	832,061	C→T	H256H (CAC→CAT)	rhlE →	RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	832,061	0	C	T	100.0%	126.0 / NA	47	H256H (CAC→CAT)	rhlE	RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/0);  total (47/0)

CCCGTACCAAACACGGCGCTAACCATCTGGCTGAACAGCTCAATAAAGATGGCATCCGTAGT  >  W3110S.gb/832048‑832109              |                                                 cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATc        >  1:670074/1‑56 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATGCGTAGt  >  1:781442/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGt     >  1:554602/1‑59 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAg   >  1:1020024/1‑61 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAg   >  1:626850/1‑61 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:675887/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:461793/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:513386/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:559635/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:560286/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:639899/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:650869/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:66999/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:318503/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:678568/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:715102/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:718590/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:797583/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:865796/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:897706/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:905961/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:917034/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:924540/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:96619/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1433214/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1022263/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1035824/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1050964/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1051656/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1092735/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1154243/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1268594/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1275558/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1293781/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1332561/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1381363/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:364836/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1465473/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:1472530/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:156137/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:163012/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:217747/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:229523/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:262609/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:280967/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:302672/1‑62 (MQ=255) cccGTACCAAACATGGCGCTAACCATCTGGCTGAACAGCTTAATAAAGATGGCATCCGTAGt  >  1:307827/1‑62 (MQ=255)              |                                                 CCCGTACCAAACACGGCGCTAACCATCTGGCTGAACAGCTCAATAAAGATGGCATCCGTAGT  >  W3110S.gb/832048‑832109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑