Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 865,327 T→C T75T (ACA→ACG)  moeB ← molybdopterin synthase sulfurylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb865,3270TC99.2% 354.9 / ‑5.1 124T75T (ACA→ACG) moeBmolybdopterin synthase sulfurylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (0/123);  minor base A (0/1);  total (0/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 124 total aligned reads displayed.

ATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCG  >  W3110S.gb/865315‑865376
            |                                                 
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGATCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:182824/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGagcagag  <  1:384828/62‑3 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:431253/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:603055/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:597793/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:596414/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:589093/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:588859/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:584971/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:563169/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:560797/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:485305/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:467497/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:456125/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:603769/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:428047/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:427979/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:427097/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:31063/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:291848/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:286457/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:259023/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:24435/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:821421/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:994812/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:993445/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:972250/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:957066/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:897397/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:894866/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:890503/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:887378/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:8529/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:841842/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:325763/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:782542/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:774886/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:734340/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:733941/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:702282/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:697875/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:669646/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:668273/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:662709/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:658806/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1151445/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1411573/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:140963/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1393244/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1381312/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1340697/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1311701/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1309550/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1278137/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1187778/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1362664/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:111232/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1097584/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1090716/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1090663/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1073847/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1057505/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1053919/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1032649/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1026205/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:237046/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:228716/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:184451/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1425303/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:17985/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:168726/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:141836/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1469597/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1427601/62‑1 (MQ=255)
aTCACTGTGCAGCGTCTGGCGTTGCAGATCGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:238911/62‑1 (MQ=255)
aTCACTGTGCAGAGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:194324/62‑1 (MQ=255)
aTCACTGTGAAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1246327/62‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1136895/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:518396/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:508146/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:489540/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1007108/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:383104/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1373032/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1097093/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1321449/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:778629/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1357113/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1156657/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:72315/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:688488/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:129375/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:627965/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGGCGAGCAGCg  <  1:155345/61‑1 (MQ=255)
 tCACTGTGCAGCGTCTGGCGTTGCAGATTAGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:391295/61‑1 (MQ=255)
  cACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:397033/60‑1 (MQ=255)
  cACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1093121/60‑1 (MQ=255)
  cACTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:1427580/60‑1 (MQ=255)
    cTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:159099/58‑1 (MQ=255)
    cTGTGCAGCGTCTGGCGTTGCAGATTGGAGAGCGAAACCGTGTCGAAGTCGAGCAGCg  <  1:576951/58‑1 (MQ=255)
            |                                                 
ATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCG  >  W3110S.gb/865315‑865376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑