Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	89,334	G→A	intergenic (+302/‑300)	fruR → / → mraZ	DNA‑binding transcriptional dual regulator/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	89,334	0	G	A	100.0%	230.3 / NA	82	intergenic (+302/‑300)	fruR/mraZ	DNA‑binding transcriptional dual regulator/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (82/0);  total (82/0)

GCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGAT  >  W3110S.gb/89281‑89342                                                      |         gcgcAAACCTGCTGTCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:46341/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGagaaga   >  1:311481/1‑61 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGagaaga   >  1:801634/1‑61 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGagaaga   >  1:798483/1‑61 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:571845/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:612190/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:567235/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:513043/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:508982/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:502700/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:500954/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:497022/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:494974/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:477342/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:446310/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:434884/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:428618/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:4092/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:403675/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:392099/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:370387/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:366827/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:811162/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:978196/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:943198/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:9225/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:919915/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:91052/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:889675/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:854069/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:846519/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:845240/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:611012/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:77332/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:741079/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:740422/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:663707/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:64855/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:631621/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:624697/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:322122/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1141835/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1334909/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1334399/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1333972/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1331760/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1287957/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1226047/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1202164/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1199961/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1189252/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1161349/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:133869/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1141711/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1137424/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1118436/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1114812/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1070551/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1065008/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1036945/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1033682/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1017781/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:241619/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:315404/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:313266/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:312250/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:307437/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:305200/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:272436/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:270308/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:258896/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:251127/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1005004/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:164139/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1471829/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:145933/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1458464/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1440018/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1391233/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1356154/1‑62 (MQ=255) gcgcAAACCTGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGAGAAGat  >  1:1349215/1‑62 (MQ=255) gcgcAAACCTGATGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCAGagaaga   >  1:566098/1‑61 (MQ=255)                                                      |         GCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGAT  >  W3110S.gb/89281‑89342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑