| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 895,685 | C→G | R91R (CGC→CGG) | potG → | putrescine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 895,685 | 0 | C | G | 99.1% | 330.1 / ‑3.6 | 116 | coding (273/1134 nt) | potG | putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (115/0); minor base . (1/0); total (116/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
GCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACG > W3110S.gb/895651‑895713 | gCGTCGATTTGTCACAGTTGCCGCCTTACCCGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1072351/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAat > 1:396698/1‑44 (MQ=38)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATatg > 1:386819/1‑47 (MQ=39)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCt > 1:864706/1‑59 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTa > 1:1356469/1‑61 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTa > 1:683725/1‑61 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTa > 1:621806/1‑61 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTa > 1:1365049/1‑61 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTa > 1:1333580/1‑61 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTa > 1:1001862/1‑61 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:620149/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:61237/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:486253/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:600868/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:599841/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:538269/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:517306/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:494197/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:493727/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:490805/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:62901/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:463412/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:447800/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:423938/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:402862/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:396179/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:386868/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:385839/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:377473/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:764605/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:969010/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:950199/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:941734/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:925239/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:914928/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:900153/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:865273/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:836831/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:828837/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:808529/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:793995/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:323977/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:75922/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:75532/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:749277/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:735689/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:731823/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:710991/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:689601/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:681145/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:672411/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:671967/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1143961/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1285336/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1284686/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:127318/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1264274/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1262269/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1253280/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1208593/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1200313/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1197266/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1187184/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1168219/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:329244/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1141952/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1116568/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1116343/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1114283/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1102948/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1076673/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1068003/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1057749/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1057635/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1045230/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:183634/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:341970/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:295532/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:295229/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:281613/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:258383/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:232589/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:222508/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:209636/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:207909/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:192616/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1314682/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:159417/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:156000/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1469534/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1463000/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1440788/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:142642/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1386623/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1373028/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1330912/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCG‑GCCATCAATATGATGTTTCAGTCTTAcg > 1:193720/1‑62 (MQ=255)gCGTCGATTTGTCACAGGTGCCGCCTTACCTGCAGCCCATCAATATGATGTTTCAGTCTTAc > 1:672209/1‑62 (MQ=255)gCGTCGATTTGTCACAGGAGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTAc > 1:1132422/1‑62 (MQ=255)gCGTCGATATGTCACAGGTGCCGCCTTACCTGCGGCCCATCAATATGATGTTTCAGTCTTa > 1:165408/1‑61 (MQ=255) | GCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACG > W3110S.gb/895651‑895713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |