Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	912,111	A→C	V154V (GTT→GTG)	hcr ←	HCP oxidoreductase, NADH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	912,111	0	A	C	100.0%	125.6 / NA	45	V154V (GTT→GTG)	hcr	HCP oxidoreductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/45);  total (0/45)

TTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGG  >  W3110S.gb/912062‑912122                                                  |            tttCCGCCACCAGCGTTACCTGATAGTTACGCCACTCATCGGCGAAAATCACATCCAgcgg  <  1:982034/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1077417/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:429495/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:495954/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:522492/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:548821/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:575459/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:608717/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:636568/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:6791/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:725942/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:752634/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:768447/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:830123/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:832021/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:839067/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:866007/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:881060/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:918113/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:94406/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:965895/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1339781/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1088528/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1100863/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1105608/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1135525/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1171089/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1264303/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1277093/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1317432/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:133399/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1334417/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:367248/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:139951/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1411149/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:240841/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:307017/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:321926/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:331517/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:338978/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:357225/61‑1 (MQ=255) tttCCGCCACCAGCGTTACCGGAAAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:547644/61‑1 (MQ=255)  ttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:783429/60‑1 (MQ=255)  ttCCGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:1421300/60‑1 (MQ=255)          ccaGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATCACATCCTgcgg  <  1:715731/52‑1 (MQ=255)                                                  |            TTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGG  >  W3110S.gb/912062‑912122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑