| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 936,162 | G→A | P839P (CCG→CCA) | ftsK → | DNA‑binding membrane protein required for chromosome resolution and partitioning |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 936,162 | 0 | G | A | 98.2% | 456.9 / ‑3.6 | 168 | P839P (CCG→CCA) | ftsK | DNA‑binding membrane protein required for chromosome resolution and partitioning |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (165/0); total (165/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
Only 100 of 170 total aligned reads displayed.
GATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCGCTGCCTTCTCTGGATTTGCTGACACCGCCGCCGAGCGAAGTGGA > W3110S.gb/936117‑936206 | gATGCGTAGTGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:1407060/1‑61 (MQ=255)gATGCGTAATGGCGCCAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTtctc > 1:496345/1‑56 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACTCCACTGCCTTCTCTGGAtt > 1:1092402/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTtctc > 1:66854/1‑56 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTtctc > 1:1453280/1‑56 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGa > 1:1077991/1‑60 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGa > 1:1412549/1‑60 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGa > 1:1469930/1‑60 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:429811/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:481933/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:478795/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:467442/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:460675/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:458650/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:451013/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:44398/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:501099/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:420741/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:415559/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:398512/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:389259/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:371170/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:355739/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:350779/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:32562/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:639421/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:764257/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:75130/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:77914/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:737276/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:728625/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:783211/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:684376/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:651831/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:792562/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:644008/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:489754/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:636692/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:62234/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:811660/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:55916/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:558336/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:551184/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:814691/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:518123/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:816883/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:909610/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:892289/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1282143/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:899679/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1220381/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1211661/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1207293/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1200858/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1177068/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1171903/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:289514/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1138921/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1120301/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1119639/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1112206/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1105303/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:925727/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:106/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1041461/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1034592/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1298397/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:323871/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:274126/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:251585/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:22897/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:221213/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:210762/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:832939/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:866538/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1424640/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1415794/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1392381/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:883847/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1353627/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1325714/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1323917/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1316444/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAtt > 1:1297566/1‑62 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:982115/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:1007147/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:750988/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:695848/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:617348/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:267191/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:203843/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:184022/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:1381806/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:1288547/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:1156726/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:1088373/1‑61 (MQ=255)gATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCACTGCCTTCTCTGGAt > 1:1008577/1‑61 (MQ=255) aTAACCCGACGACGCCGCTGCCTTCTCTGGATTTGCTGACACCGCCGCCGCGCGAAGTGGa < 1:1281290/61‑1 (MQ=255) aTAAACCGACGACGCCGCTGCCTTCTCTGGATTTGCTGACACCGCCGCCGAGCGAAGTGGa < 1:64735/61‑1 (MQ=255) acgacgCCGCTGCCTTCTCTGGATTTGCTGACACCGCCGCCGAGCGAAGTGGa < 1:523505/53‑1 (MQ=255) | GATGCGTAATGGCGACAGCCGTCCGTTGCATAAACCGACGACGCCGCTGCCTTCTCTGGATTTGCTGACACCGCCGCCGAGCGAAGTGGA > W3110S.gb/936117‑936206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |