BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	947,132	C→T	I280I (ATC→ATT)	ycaD →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	947,132	0	C	T	100.0%	329.0 / NA	118	I280I (ATC→ATT)	ycaD	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/118); total (0/118)

Only 100 of 118 total aligned reads displayed.

TGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCC  >  W3110S.gb/947111‑947145
                     |
tGTCGTCATTCTCTGCAGTATTGCGATGCTTAGcc  <  1:1199384/35‑1 (MQ=37)
tGTCGTCATTCTCTGCAGTATTGCGATGCTTAGcc  <  1:1147614/35‑1 (MQ=37)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTATcc  <  1:52168/35‑1 (MQ=25)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTATcc  <  1:518667/35‑1 (MQ=25)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGct  <  1:1343745/35‑2 (MQ=37)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:470620/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:585998/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:553662/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:540872/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:529691/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:528526/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:520296/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:506643/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:498924/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:605879/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:45951/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:435034/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:430779/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:413147/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:409109/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:401449/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:399744/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:347917/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:347229/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:334399/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:301779/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:296196/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:781078/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:997280/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:963639/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:954881/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:945653/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:944650/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:923225/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:872054/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:841677/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:835434/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:820751/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:819570/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:794704/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:586450/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:755565/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:743710/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:726918/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:714556/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:707881/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:706848/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:680132/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:670801/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:66260/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:649661/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:292646/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1121629/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:295995/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1253456/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1250890/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1235619/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1211823/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1211645/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:118996/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1174371/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1168821/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1155171/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1138628/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1258411/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1117486/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1090456/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:108668/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1077926/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1062681/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1058422/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1056818/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1030024/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1017585/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:101287/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1009988/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1310983/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:287679/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:280651/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:23802/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:236382/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:23541/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:193769/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:184431/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:174256/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:164563/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1470385/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1466487/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1445600/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1442353/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1428844/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1425155/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1378674/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1359949/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1321347/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1320117/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1316335/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1277626/35‑1 (MQ=255)
tGTCGTCATTCTCGGCAGTATTGCGATGCTCAGcc  <  1:1003479/35‑1 (MQ=25)
tGTAGTCATTCTCGGCAGTATTGCGATGCTTAGcc  <  1:1020811/35‑1 (MQ=25)
                     |
TGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCC  >  W3110S.gb/947111‑947145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`