Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	966,404	C→T	L555L (CTG→TTG)	ycaI →	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	966,404	0	C	T	100.0%	135.3 / NA	50	L555L (CTG→TTG)	ycaI	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/0);  total (50/0)

TATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATC  >  W3110S.gb/966358‑966419                                               |                tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACAt   >  1:1197555/1‑61 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACAt   >  1:778008/1‑61 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:736955/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:507141/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:511740/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:524485/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:532106/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:587249/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:59718/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:623151/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:650938/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:671324/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:676621/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:684558/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:713062/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:471193/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:779739/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:802737/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:846873/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:868432/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:875820/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:884330/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:895498/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:911313/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:933106/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:954426/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1420874/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1076985/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1080991/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1097335/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1114923/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1123018/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1150997/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1179357/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1279601/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1301532/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1337290/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1417971/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1068764/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1429347/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:1455510/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:148607/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:152163/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:250223/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:28152/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:295810/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:333685/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:382338/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:431584/1‑62 (MQ=255) tATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTTTGAGTCATGAACATc  >  1:435411/1‑62 (MQ=255)                                               |                TATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATC  >  W3110S.gb/966358‑966419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑