Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	991,289	T→C	V82V (GTT→GTC)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	991,289	0	T	C	100.0%	164.9 / NA	59	V82V (GTT→GTC)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (59/0);  total (59/0)

TGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTTATCAGTAATTTGCCGGAGCGTTTTAC  >  W3110S.gb/991254‑991315                                    |                           tGGACCGCCTGGATAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1464537/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGcc              >  1:475111/1‑50 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCgg            >  1:696843/1‑52 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGtttt    >  1:1255693/1‑60 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGt       >  1:1240529/1‑57 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTa   >  1:885727/1‑61 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:768108/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:776262/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:717352/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:684853/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:626028/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:625506/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:619260/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:594340/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:579346/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:549739/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:497982/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:487120/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:772710/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:474838/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:794767/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:804332/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:81170/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:812826/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:826965/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:829464/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:842986/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:845190/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:875104/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:876825/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:882400/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:910189/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1345824/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1029007/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1067703/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1090922/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1106772/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1129851/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1156830/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1169917/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1224993/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1264816/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1314316/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1322588/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1342062/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:474940/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1395264/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1413556/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1426789/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1435001/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:204260/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:341671/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:342944/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:375901/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:417925/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:454868/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:470750/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGAGCGTTTTAc  >  1:1028125/1‑62 (MQ=255) tGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTCATCAGTAATTTGCCGGACCGTTTTAc  >  1:114825/1‑62 (MQ=255)                                    |                           TGGACCGCCTGGAAAGAAGAAGAGGGCGCACTGGTTATCAGTAATTTGCCGGAGCGTTTTAC  >  W3110S.gb/991254‑991315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑