Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	993,984	C→A	G161G (GGG→GGT)	ssuB ←	alkanesulfonate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	993,984	0	C	A	100.0%	215.7 / NA	79	G161G (GGG→GGT)	ssuB	alkanesulfonate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (79/0);  total (79/0)

GGCGTCCAGCGCCCCGAGCGGTTCATCAAGCAACAATAATCCCGGTCGATGAATCAACGCTC  >  W3110S.gb/993972‑994033             |                                                  agCGTCCAGCGCACCGAGTGGTTCATCAAGCTACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1123055/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACaata                          >  1:1281597/2‑38 (MQ=25) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACaata                          >  1:891699/2‑38 (MQ=25) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:498458/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:680293/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:678549/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:634394/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:633985/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:612784/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:60333/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:593777/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:546677/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:544659/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:686088/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:486736/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:485537/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:460121/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:454760/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:420653/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:416351/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:414015/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:410527/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:879519/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:988123/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:986399/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:975726/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:966226/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:960560/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:956581/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:921019/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:879784/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:684579/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:866371/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:840986/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:83312/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:788102/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:786889/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:759984/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:727185/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:343868/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1233710/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1355115/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1354616/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1325957/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1306752/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1292052/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1271961/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1265730/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:126111/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1236178/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1360776/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1222620/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1176261/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1157394/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1141478/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1130390/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1110172/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1101696/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1097902/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1057309/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1364846/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1056128/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:335069/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:33395/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:290919/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:289694/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:246438/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:241129/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:238994/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:197294/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:192128/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:147442/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1455019/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1423901/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1401159/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1398836/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:1382253/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgccc  >  1:379912/2‑60 (MQ=255) agCGTCCAGCGCACCGAGTGGTTCATCAAGCAACAATAAACCTGGTCGATGAATCAgcgcca  >  1:511730/2‑60 (MQ=255)             |                                                  GGCGTCCAGCGCCCCGAGCGGTTCATCAAGCAACAATAATCCCGGTCGATGAATCAACGCTC  >  W3110S.gb/993972‑994033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑