Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 94,793 T→C F48F (TTT→TTC)  murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb94,7930TC100.0% 318.9 / NA 112F48F (TTT→TTC) murFUDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (112/0);  total (112/0)

Only 100 of 112 total aligned reads displayed.

CGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTG  >  W3110S.gb/94753‑94809
                                        |                
ctccGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:653523/3‑57 (MQ=255)
cgcgGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:896560/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGa       >  1:473265/1‑52 (MQ=38)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGAtttt   >  1:455044/1‑56 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGAtttt   >  1:774878/1‑56 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGAtttt   >  1:807241/1‑56 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGAtttt   >  1:335121/1‑56 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGAtttt   >  1:330904/1‑56 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGAtttag  >  1:141028/1‑55 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGAttt    >  1:1264318/1‑55 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGAt      >  1:89257/1‑53 (MQ=38)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:721737/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:71892/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:692324/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:761795/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:678311/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:674684/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:665716/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:725349/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:643248/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:640579/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:613810/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:601582/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:529446/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:527870/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:523998/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:516058/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:507108/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:504856/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:858418/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:994988/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:978549/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:954677/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:940830/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:940623/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:93055/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:923608/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:886862/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:87598/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:875372/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:869937/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:736131/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:840299/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:813391/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:80963/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:804749/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:803114/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:782272/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:764800/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:471560/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:746044/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:738655/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:122045/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:49672/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1440887/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1436480/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1424265/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1403005/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1402470/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1396635/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1320544/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1291717/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1289258/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1280436/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:160725/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1213185/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1212842/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:117150/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1139594/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1139079/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1114236/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1103775/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1072714/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1049951/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:104842/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1029786/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:315751/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:1008345/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:457838/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:441451/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:428391/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:422143/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:401098/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:39723/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:388878/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:35858/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:356730/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:161842/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:306046/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:30433/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:289134/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:2441/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:212495/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:211977/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:211407/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:195212/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:185116/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTGCCCTGAAACGCGAACGTTTCGATGCTCATGATTTTg  >  1:745483/1‑57 (MQ=255)
cGCCGGGCTGCCTGTTTGTTACCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:929050/1‑57 (MQ=255)
cGCCGGGCAGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTCGATGCTCATGATTTTg  >  1:240327/1‑57 (MQ=255)
                                        |                
CGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTG  >  W3110S.gb/94753‑94809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑