Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,085,807 G→A G132S (GGC→AGC)  phoH → conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,085,8070GA83.4% 303.3 / 41.9 163G132S (GGC→AGC) phoHconserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base G (0/27);  new base A (136/0);  total (136/27)
Fisher's exact test for biased strand distribution p-value = 1.99e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

Only 100 of 164 total aligned reads displayed.

CAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAGCCATTGGTATG  >  W3110S.gb/1085758‑1085830
                                                 |                       
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:164691/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:542736/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:86409/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:1237955/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:18662/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:1177532/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:1173102/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:554299/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:1159998/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:898791/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:561983/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:450032/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:421467/62‑1 (MQ=255)
cAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:1019224/62‑1 (MQ=255)
 aGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:924165/61‑1 (MQ=255)
 aGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:339785/61‑1 (MQ=255)
 aGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:489581/61‑1 (MQ=255)
 aGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAg             <  1:652501/61‑1 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGcc           >  1:765376/1‑53 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTAt   >  1:38569/1‑61 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTAt   >  1:1456661/1‑61 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTAt   >  1:230082/1‑61 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTAt   >  1:1161320/1‑61 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:503121/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:533924/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:493485/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:465300/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:459300/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:454699/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:562151/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:452815/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:69741/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:577971/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:615413/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:84763/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:971623/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:95191/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:948528/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:943601/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:930601/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:919092/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:898618/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:897268/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:874931/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:856202/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:624280/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:830070/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:812704/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:790687/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:750198/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:747071/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:732619/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:320727/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:689528/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:659427/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:647874/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1224881/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:389589/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1347468/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1338637/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1320351/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:130731/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1301936/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1297067/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1278260/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1276606/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1251263/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1352275/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1203373/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1156678/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1133721/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1131590/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1114301/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1101930/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1070380/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1043709/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:100923/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:188958/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:356518/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:342801/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:322734/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1006587/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:256709/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:244924/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:241007/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:217196/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:206534/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:198819/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1359366/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:182726/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:159129/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1461949/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1423331/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1402476/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1399394/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1397678/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1393872/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1387989/1‑62 (MQ=255)
           agcGTGAAGAAGAATCGGTCACCTCGCTTGTGCACATGAGCGGCGTAGAAg             >  1:1332984/1‑51 (MQ=255)
           agcGTGAAGAACAATCGGTCACCTCGCTTGTGCAGATGAGCGGCGTAGAAGCCATTGGTATg  >  1:1019821/1‑62 (MQ=255)
                                                 |                       
CAGCCATAAGCAGCGTGAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAGCCATTGGTATG  >  W3110S.gb/1085758‑1085830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑