Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,109,252 G→T A441S (GCA→TCA)  ymdC → predicted hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,109,2520GT83.2% 262.1 / 48.0 125A441S (GCA→TCA) ymdCpredicted hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/21);  new base T (104/0);  total (104/21)
Fisher's exact test for biased strand distribution p-value = 2.79e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 125 total aligned reads displayed.

GCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTA  >  W3110S.gb/1109198‑1109267
                                                      |               
ggcTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:428544/59‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:416072/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:668411/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:1354201/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:136171/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:590726/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:797054/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:1170601/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:1166849/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:461560/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:459028/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:309884/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:950887/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:454895/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:949102/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGGCATGCaaaga           <  1:251960/61‑1 (MQ=255)
gCCTGGCAGCTCCGTCAGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:1318536/61‑1 (MQ=255)
gCCTGGAAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaaga           <  1:805769/61‑1 (MQ=255)
  cTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaagga           <  1:1041650/59‑1 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:526402/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:539878/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:553371/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:555203/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:560677/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:574502/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:590254/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:512936/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:433664/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:492731/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:702092/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:659867/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:468624/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:855407/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:982480/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:962057/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:961489/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:959786/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:950125/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:948704/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:936609/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:927861/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:919157/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:913313/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:666457/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:850081/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:827664/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:814226/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:809834/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:784741/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:749291/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:718329/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:707049/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:292881/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:693692/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1112539/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1284640/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1273677/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1261491/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1257600/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1251137/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1248698/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1206711/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1206544/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1125272/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1125095/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1113301/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1296268/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1095906/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:107269/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1064891/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1059543/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1043091/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:104118/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:102589/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1020306/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1013121/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1012253/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1470912/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:391148/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:354280/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:100917/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:281630/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:27689/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:23555/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:224696/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:220856/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:181079/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:179594/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:149415/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1447918/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1413018/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1389977/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1387859/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1363716/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1352840/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1316628/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1315339/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1305931/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatta  >  1:1299207/1‑52 (MQ=255)
                  gACAGGTGGGGACGGATCAACTGGGTTGATCGTCATTCAAAGAAAGAGatt   >  1:408966/1‑51 (MQ=255)
                                                      |               
GCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTA  >  W3110S.gb/1109198‑1109267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑