Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,118,473 C→T Y30Y (TAC→TAT)  yceA → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,118,4730CT100.0% 367.5 / NA 131Y30Y (TAC→TAT) yceAconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/131);  total (0/131)

Only 100 of 131 total aligned reads displayed.

AGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACA  >  W3110S.gb/1118425‑1118486
                                                |             
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:546736/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:687639/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:676231/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:6737/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:66584/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:653786/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:647907/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:607223/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:595427/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:572621/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:561285/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:551219/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:688184/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:532125/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:515191/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:486090/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:482852/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:468009/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:464252/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:458392/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:42042/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:406137/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1001054/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:370221/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:8438/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:981987/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:974039/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:973924/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:946965/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:941812/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:937611/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:904858/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:892426/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:890836/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:884143/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:873377/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:393861/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:800629/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:770371/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:766115/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:759790/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:74237/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:734090/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:730555/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:718574/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:716239/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:706263/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:694145/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1154357/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:129982/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:349234/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1279487/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1232670/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1187237/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1187129/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1181882/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1172850/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1169041/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1158019/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1157579/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1279637/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1139877/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1122881/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1114255/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1107938/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1093348/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1086769/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1086598/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1072900/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:10294/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1029174/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1003569/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1305171/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:340048/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:284801/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:213572/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:157594/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:153174/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:144611/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1433928/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:14169/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1394091/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1378368/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1372513/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1304037/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:134819/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:134682/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:131670/62‑1 (MQ=255)
aGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTACTTCcaca  <  1:251005/62‑1 (MQ=255)
 gCCGAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:640399/61‑1 (MQ=255)
 gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:385016/61‑1 (MQ=255)
 gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:309789/61‑1 (MQ=255)
 gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1065647/61‑1 (MQ=255)
 gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:289539/61‑1 (MQ=255)
 gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1215818/61‑1 (MQ=255)
 gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:495583/61‑1 (MQ=255)
 gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1357871/61‑1 (MQ=255)
 gCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:745783/61‑1 (MQ=255)
        tGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:43753/54‑1 (MQ=255)
              cTGAGAGCGAACCGCGAACCACCATTTCGTTTTATAAGTATTTCcaca  <  1:1075981/48‑1 (MQ=255)
                                                |             
AGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACA  >  W3110S.gb/1118425‑1118486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑