Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,132,336	T→C	Q35Q (CAA→CAG)	flgA ←	assembly protein for flagellar basal‑body periplasmic P ring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,132,336	0	T	C	100.0%	269.6 / NA	94	Q35Q (CAA→CAG)	flgA	assembly protein for flagellar basal‑body periplasmic P ring
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/94);  total (0/94)

GAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGC  >  W3110S.gb/1132304‑1132341                                 |      taaaCACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:434502/37‑1 (MQ=38) gAAACACGTACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:906962/38‑1 (MQ=38) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:569933/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:760631/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:723955/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:717350/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:711246/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:710275/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:701624/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:666833/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:642698/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:642297/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:599389/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:599067/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:772288/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:565398/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:550831/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:539596/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:525681/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:522123/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:509024/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:486462/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:469690/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1003277/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:868851/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:980853/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:979258/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:979079/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:974278/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:964247/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:958898/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:903389/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:899726/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:891506/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:891418/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:876518/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:770429/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:863880/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:854535/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:850646/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:83337/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:826334/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:81224/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:796273/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:794127/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:778867/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:462911/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1307963/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:445773/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1424470/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1393225/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1389031/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1381617/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1368117/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1347815/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:133690/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1335760/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1332972/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1332395/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1431801/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:12476/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1242209/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1238151/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1117889/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1081628/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1075062/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1073409/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1063834/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:103073/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1018592/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:263866/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:429381/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:422720/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:422353/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:397882/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:392699/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:388308/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:373784/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:36650/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:311641/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1451155/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:25529/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:232756/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:211739/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:17782/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:160827/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:152444/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1462851/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1460391/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:1459377/38‑1 (MQ=255) gAAACACGAACCTCATCACTTACCCCCGCGAGCTGAgc  <  1:285822/38‑1 (MQ=38)  aaaCACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:868020/37‑1 (MQ=255)  aaaCACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:387462/37‑1 (MQ=255)  aaaCACGAACCTCATCACTTACCCCCGCGAGCTGGgc  <  1:786065/37‑1 (MQ=255)                                 |      GAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGC  >  W3110S.gb/1132304‑1132341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑