Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,254,608	T→C	intergenic (+46/+54)	dhaR → / ← ycgV	predicted DNA‑binding transcriptional regulator, dihydroxyacetone/predicted adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,254,608	0	T	C	100.0%	28.2 / NA	12	intergenic (+46/+54)	dhaR/ycgV	predicted DNA‑binding transcriptional regulator, dihydroxyacetone/predicted adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/0);  total (12/0)

CGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCA  >  W3110S.gb/1254554‑1254616                                                       |         cGGGTATGAATGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:1156345/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGGTACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:461534/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:1138840/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:1278103/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:129645/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:130658/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:1383704/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:1392914/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:541322/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcgc   >  1:673185/1‑62 (MQ=255) cGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGgcg    >  1:1334872/1‑61 (MQ=255) cGGGTATG‑AAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCa  >  1:1034952/1‑62 (MQ=255)                                                       |         CGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCA  >  W3110S.gb/1254554‑1254616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑