Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,268,022	A→G	T118A (ACG→GCG)	prmC →	N5‑glutamine methyltransferase, modifies release factors RF‑1 and RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,268,022	0	A	G	97.5%	107.8 / ‑3.1	40	coding (352/834 nt)	prmC	N5‑glutamine methyltransferase, modifies release factors RF‑1 and RF‑2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (39/0);  minor base . (1/0);  total (40/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCG  >  W3110S.gb/1267986‑1268048                                     |                           ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:870908/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:1023214/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:774624/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:788394/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:818611/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:821535/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:827205/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:828985/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:833363/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:840118/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:704792/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:898413/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:900081/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:961688/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:962876/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:963454/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:971427/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:987013/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:988850/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:994481/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:151798/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:1050564/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:1055722/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:1060886/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:1065658/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:1132241/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:1299924/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:1396364/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:154369/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:31196/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:325431/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:328445/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:398212/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:399905/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttgc   >  1:46349/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttg    >  1:276169/1‑61 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttg    >  1:1379682/1‑61 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGAttgcgcttg    >  1:48005/1‑61 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCGCGTACCGGGGCGAttgcgcttgc   >  1:545934/1‑62 (MQ=255) ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGG‑GGGGTACCGGGGCGAttgcgcttgcg  >  1:899856/1‑62 (MQ=255)                                     |                           TTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCG  >  W3110S.gb/1267986‑1268048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑