Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,310,802	T→G	S394S (TCA→TCC)	kch ←	voltage‑gated potassium channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,310,802	0	T	G	100.0%	99.2 / NA	36	S394S (TCA→TCC)	kch	voltage‑gated potassium channel
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/0);  total (36/0)

CCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTAAAA  >  W3110S.gb/1310741‑1310857                                                              |                                                        ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATTGCTGAAAATACCATGACCt                                                         <  1:349008/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:601317/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:233239/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:279185/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:292126/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:30629/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:377180/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:401440/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:431015/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:435518/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:460893/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:464845/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:49651/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:501148/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:578675/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:5798/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1008784/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:613097/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:622515/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:632077/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:675781/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:738795/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:763090/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:76767/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:936543/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:939359/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:949902/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:956672/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:966858/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:976602/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:99486/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1199436/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1181774/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1277801/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1281790/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:148674/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1282902/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1062181/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1305015/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1305216/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1414387/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1353868/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1352039/62‑1 (MQ=255) ccGATTCTTTACTGTCAGCTTTCGTTTCTAGCTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1315801/62‑1 (MQ=255)  cGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:622980/61‑1 (MQ=255)  cGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:1133428/61‑1 (MQ=255)              gTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                         <  1:441659/49‑1 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTTAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:877328/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1005785/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:826985/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:840605/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1074608/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1052998/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:785189/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1043166/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1150639/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1041050/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:976074/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:729481/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:693667/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1031586/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:993415/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:180968/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1426915/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:244252/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:171324/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:291948/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:170347/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:299090/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:30440/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:330285/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:333302/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:648612/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1392636/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:450828/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:525276/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:54214/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:553542/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1231661/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1225563/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaaaa  >  1:1209197/1‑62 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTaa    >  1:1088221/1‑60 (MQ=255)                                                        aTGACCGGAGTTCAACAACATTGAAACGAGCAAATCATTATTAATCTCTTCACCATTTaaaa  >  1:341242/1‑62 (MQ=255)                                                              |                                                        CCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCATTTAAAA  >  W3110S.gb/1310741‑1310857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑