Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,359,069 G→T intergenic (‑245/+68) sapA ← / ← ymjA predicted antimicrobial peptide transporter subunit/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,359,0690GT100.0% 334.6 / NA 121intergenic (‑245/+68)sapA/ymjApredicted antimicrobial peptide transporter subunit/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (121/0);  total (121/0)

Only 100 of 121 total aligned reads displayed.

GAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAAC  >  W3110S.gb/1359034‑1359090
                                   |                     
gaACCCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:616134/1‑57 (MQ=25)
gaAACCCTTTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:72931/1‑57 (MQ=25)
gaAACCCATTAAAGGATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1400981/1‑57 (MQ=25)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGccgg               >  1:1312483/1‑42 (MQ=38)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGccgg               >  1:566608/1‑42 (MQ=38)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGccgg               >  1:892017/1‑42 (MQ=38)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCTGGATGCGGCGCGAAc  >  1:923437/1‑57 (MQ=255)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATgc           >  1:1205004/1‑48 (MQ=255)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGaa   >  1:1135272/1‑56 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGa    >  1:373992/1‑55 (MQ=255)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:574597/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:5968/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:600415/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:55477/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:530274/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:606117/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:505700/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:515812/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:655873/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:490358/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:448538/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:386039/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:381372/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:377205/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:369523/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:346200/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:345575/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:340098/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:339274/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:793802/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:956446/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:947263/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:933789/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:908687/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:906990/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:903731/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:897212/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:85435/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:815646/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:806364/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:80369/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:623951/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:747575/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:706775/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:69998/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:690626/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:683553/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:674538/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:670303/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:663429/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:311706/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:625226/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1082978/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1239719/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1238182/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:123215/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1230922/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1222502/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1163244/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1161394/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:115629/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1131505/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1127930/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1127186/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1262955/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1068694/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1060494/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1048644/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1046481/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1042543/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1039680/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1032801/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1017896/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1013003/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1012450/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1006836/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1439861/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:318902/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1002990/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:287075/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:28424/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:251586/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:240397/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:231692/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:207151/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:177183/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1472260/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1451315/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:328565/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1434706/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1421922/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1417544/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1371301/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1366920/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1346846/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1332478/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1322275/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1270660/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGCCGGATGCGGCGCGAAc  >  1:1270032/1‑57 (MQ=37)
gaAACCCATTAAAGCATGCTGCAGTGCGTGTCATTTTTAGACGGATGCGGCGCGAAc  >  1:1296378/1‑57 (MQ=255)
                                   |                     
GAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAAC  >  W3110S.gb/1359034‑1359090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑