Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,383,822 A→T S54S (TCA→TCT)  ompG → outer membrane porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,383,8220AT88.9% 194.7 / 13.7 81S54S (TCA→TCT) ompGouter membrane porin
Reads supporting (aligned to +/- strand):  ref base A (9/0);  new base T (72/0);  total (81/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAA  >  W3110S.gb/1383770‑1383856
                                                    |                                  
tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                           >  1:1307334/1‑62 (MQ=255)
tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                           >  1:371113/1‑62 (MQ=255)
tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                           >  1:36751/1‑62 (MQ=255)
tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                           >  1:616337/1‑62 (MQ=255)
tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                           >  1:1357156/1‑62 (MQ=255)
tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                           >  1:1353977/1‑62 (MQ=255)
tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                           >  1:663019/1‑62 (MQ=255)
tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                           >  1:1097060/1‑62 (MQ=255)
tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                           >  1:1122073/1‑62 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGcc          >  1:1305929/1‑39 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCg         >  1:591028/1‑40 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCg         >  1:848985/1‑40 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTg       >  1:98741/1‑42 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:985481/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:622494/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:590553/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:555895/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:549981/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:524205/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:490649/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:485196/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:482185/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:43599/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:409470/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:394227/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:39185/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:389622/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:38429/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:723105/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:922339/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:912745/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:90786/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:861297/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:837518/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:835144/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:809390/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:742993/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:617274/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:71831/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:689541/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:337183/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:64217/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:949682/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:956122/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:937639/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1184664/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1259012/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:12526/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1250546/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:12410/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1230839/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1205895/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1198889/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1192454/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1292164/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1182541/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1174477/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1138982/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1123272/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1108519/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1107003/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:110221/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1045170/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:197377/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:342417/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1028213/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:326379/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:290558/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:267069/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:262048/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:232297/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:224718/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:35223/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:189804/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:159706/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1464745/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1452975/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1406358/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1396554/1‑47 (MQ=255)
                                        ggCGGAGCCTTCTGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1370944/1‑47 (MQ=255)
                                        ggCGGAGCCTTATGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa  >  1:1321403/1‑47 (MQ=39)
                                                    |                                  
TAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAA  >  W3110S.gb/1383770‑1383856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑