Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,441,263	C→T	L412L (CTG→CTA)	ydbK ←	fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,441,263	0	C	T	94.3%	82.5 / ‑3.2	35	L412L (CTG→CTA)	ydbK	fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (0/33);  total (0/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTCCAGTTTCGCCGAGTTTGGCAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTCACATCATCGT  >  W3110S.gb/1441242‑1441309                      |                                               ttCCAGTTTCGCCGAGTTTGGCAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTCAcat        <  1:1092351/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:518833/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:955288/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:554179/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:602297/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:603973/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:605197/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:637828/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:73260/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:795231/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:796479/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:801918/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:815994/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:848661/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:917135/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:930077/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:936342/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:1045272/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:159373/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:1124016/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:1217109/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:1307766/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:1308282/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:1408023/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:1466476/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:1468870/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:157606/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:464878/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:289887/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:306199/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:385810/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:405180/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:411789/62‑1 (MQ=255)       tttCGCCGAGTTTGGTAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:416234/62‑1 (MQ=255)       tttCGCCGAGTTTGGCAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTTACATCATCGt  <  1:20708/62‑1 (MQ=255)                      |                                               TTCCAGTTTCGCCGAGTTTGGCAGGGTGTTTTCCGGCAACGGCAGTGACAGATTGGTCACATCATCGT  >  W3110S.gb/1441242‑1441309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑