Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,526,615	T→C	L360L (CTA→CTG)	ansP ←	L‑asparagine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,526,615	0	T	C	100.0%	244.2 / NA	86	L360L (CTA→CTG)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (86/0);  total (86/0)

AGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACA  >  W3110S.gb/1526584‑1526645                                |                               aggtGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1265734/4‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGaa                   >  1:1334922/1‑45 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGaa                   >  1:639035/1‑45 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAAt                  >  1:1008325/1‑46 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGTcaca  >  1:383226/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:415193/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:306136/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:467475/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:460043/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:438277/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:429458/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:426647/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:416699/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:967605/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:312497/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:314626/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:400216/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:390858/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:389115/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:376616/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:375422/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:360377/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:327078/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:315639/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:724091/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:100464/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:953496/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:92496/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:914591/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:900012/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:868973/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:860035/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:846487/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:830665/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:492088/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:681377/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:676179/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:65826/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:608598/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:595535/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:59541/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:571864/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:542045/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1158138/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1327742/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:283513/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:129485/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1284023/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1260320/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1181067/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1179995/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1179578/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1159804/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1318280/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1133120/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1131473/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1129120/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1107255/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:110293/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1095798/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1045030/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1027756/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:157631/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:283288/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:267951/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:24365/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:215543/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:212017/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:2098/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:189384/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:187951/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1327897/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1461431/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1458373/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1423494/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1420205/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1353775/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1350426/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1348127/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcaca  >  1:1340755/1‑62 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcac   >  1:839036/1‑61 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcac   >  1:643823/1‑61 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcac   >  1:1397180/1‑61 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcac   >  1:910937/1‑61 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcac   >  1:1310383/1‑61 (MQ=255) aGTTGAGGAATACGCCGACGACATACACAACCAGTGTCGCCAGAATCCCGGCATACGGcac   >  1:926996/1‑61 (MQ=255)                                |                               AGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACA  >  W3110S.gb/1526584‑1526645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑