Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	130,018	T→C	T415A (ACA→GCA)	yacH ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	130,018	0	T	C	100.0%	34.6 / NA	14	T415A (ACA→GCA)	yacH	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/14);  total (0/14)

GGCGTGTGTTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTGGG  >  W3110S.gb/130010‑130070         |                                                     gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:1162662/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:143479/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:145309/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:199485/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:202448/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:78405/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:849830/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:883872/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:922663/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:935991/59‑1 (MQ=255) gacgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:943982/59‑1 (MQ=255)  acgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:308292/59‑1 (MQ=255)  acgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:551750/59‑1 (MQ=255)  acgTGTGCTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTggg  <  1:908236/59‑1 (MQ=255)         |                                                     GGCGTGTGTTCGTTGCTGAAACTGATTTGCTGCCGCCTGACGCTGGCTGTCGCGCGTTGGG  >  W3110S.gb/130010‑130070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑