Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,551,751	G→A	A879A (GCG→GCA)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,551,751	0	G	A	100.0%	114.6 / NA	43	A879A (GCG→GCA)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/43);  total (0/43)

TAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCG  >  W3110S.gb/1551718‑1551809                                  |                                                           tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:676215/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:376854/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:385877/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1066748/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:458079/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:471810/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:540242/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:577569/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:609543/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:630600/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:655703/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:449614/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:72264/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:732168/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:768543/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:896863/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:902581/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:905240/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:910026/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:946799/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:997677/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1317197/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1120428/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1192437/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1204329/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1210682/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1219917/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1242022/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1275744/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1280597/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:285468/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1361846/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1443119/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:146834/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:170040/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:234891/62‑1 (MQ=255) tAACCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:264400/62‑1 (MQ=255)  aaCCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:631465/61‑1 (MQ=255)  aaCCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1247879/61‑1 (MQ=255)  aaCCCGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:780736/61‑1 (MQ=255)    cccGGTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:21149/59‑1 (MQ=255)       ggTTGTTCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:946737/56‑1 (MQ=255)         ttgttCGTCTGTATGAACAAGACGCACTGCGGATGGGTAAAAAAGAGCAGTTcc                                <  1:1396245/54‑1 (MQ=255)                               cgcgCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTAc                   >  1:534728/1‑45 (MQ=255)                               cgcgCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg  >  1:1339657/1‑62 (MQ=255)                               cgcgCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg  >  1:584/1‑62 (MQ=255)                               cgcgCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg  >  1:913681/1‑62 (MQ=255)                                  |                                                           TAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCG  >  W3110S.gb/1551718‑1551809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑