Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,587,262	C→T	E313E (GAG→GAA)	ydeP ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,587,262	0	C	T	100.0%	196.7 / NA	71	E313E (GAG→GAA)	ydeP	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/71);  total (0/71)

GAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAA  >  W3110S.gb/1587211‑1587272                                                    |           gagcACGTCACGGCGTAGCTCGTCAAAGCCGAGGGTATGCGTTTGAATAAATTCGtcatcca  <  1:161670/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:570964/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:655899/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:651707/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:650796/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:630682/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:618731/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:607949/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:575922/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:664743/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:546681/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:536385/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:523831/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:493184/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:469548/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:45910/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:455096/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:441943/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:820071/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:960619/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:941066/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:938537/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:932487/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:920284/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:908/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:900235/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:83429/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1052418/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:816258/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:810895/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:776455/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:759530/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:690345/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:669146/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:665270/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:392964/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1029204/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1062886/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1164039/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1167574/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1213671/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1225695/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1306700/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1313015/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1328157/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1329077/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1367105/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:138735/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:139429/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1394996/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1427384/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:225643/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:298478/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:297153/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:274484/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:244787/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:2430/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:242729/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:213649/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:357045/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:170422/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:169652/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:430393/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:164262/62‑3 (MQ=255) gagcACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:148785/62‑3 (MQ=255) gagcACGTCACGGCGTAGCCCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:911378/62‑3 (MQ=255)       gTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:286620/56‑3 (MQ=255)          aCGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:972613/53‑3 (MQ=255)                 aGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:176464/46‑3 (MQ=255)                   ctctTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:683860/44‑3 (MQ=255)                     cGTCAAAGCCGACGGTATGCGTTTGAATAAATTCGtcatcca  <  1:1427437/42‑3 (MQ=255)                                                    |           GAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAA  >  W3110S.gb/1587211‑1587272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑