Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,611,870 G→A Y175Y (TAC→TAT)  uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,611,8700GA100.0% 446.8 / NA 158Y175Y (TAC→TAT) uxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (158/0);  total (158/0)

Only 100 of 158 total aligned reads displayed.

CCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGG  >  W3110S.gb/1611845‑1611890
                         |                    
ccAGTTCACGCAACGCGTCGCCATTATAGtcaatca            >  1:1028587/1‑36 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTca       >  1:1424536/1‑41 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:379861/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:544780/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:535612/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:526709/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:487594/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:485885/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:453015/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:432535/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:417966/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:417841/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:408275/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:406294/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:561038/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:379335/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:376316/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:363771/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:359651/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:34721/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:324819/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:279213/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1005571/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:275537/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:265021/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:775042/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:983579/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:957936/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:933408/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:913478/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:912078/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:904070/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:896315/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:814663/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:803592/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:794376/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:777380/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:279106/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:756566/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:752558/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:748814/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:736135/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:729830/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:706889/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:687912/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:657570/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:632461/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:621646/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1109187/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1289136/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1289118/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:258379/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:127158/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1261909/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1258502/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:123930/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1222773/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1203035/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1191195/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1144363/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1272302/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1103046/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1102870/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1088755/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1083000/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1064020/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1062553/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1061953/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1043691/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:103417/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1029577/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1017947/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:16085/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:247922/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:245564/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:238188/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:228968/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:217685/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:215786/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:211559/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:208145/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:202492/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:174342/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1312816/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1451211/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1448476/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1443955/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1402927/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1377047/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:1373715/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:134671/1‑46 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACg   >  1:146618/1‑45 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACg   >  1:843216/1‑45 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACg   >  1:851523/1‑45 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACg   >  1:880803/1‑45 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACg   >  1:718989/1‑45 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACg   >  1:646236/1‑45 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTATAGTCAATCAACTCACACg   >  1:1322629/1‑45 (MQ=255)
ccAGTTCACGCAACGCGTCGCCATTAAAGTCAATCAACTCACACg   >  1:463831/1‑45 (MQ=38)
ccAGTTAACGCAACGCGTCGCCATTATAGTCAATCAACTCACACgg  >  1:528369/1‑46 (MQ=38)
                         |                    
CCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGG  >  W3110S.gb/1611845‑1611890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑