| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,615,593 | A→G | I275I (ATT→ATC) | yneI ← | predicted aldehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,615,593 | 0 | A | G | 84.4% | 250.5 / 28.2 | 129 | I275I (ATT→ATC) | yneI | predicted aldehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (10/10); new base G (0/108); total (10/119) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.14e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
GCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCT > W3110S.gb/1615543‑1615647 | gCAGCTGCCACAAAACGTTCGGTATATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:207513/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATTATAAAGCGttt < 1:1161109/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATACAGCGttt < 1:1368606/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:44055/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:538476/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:515384/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:505589/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:50298/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:490733/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:476229/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:468673/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:465503/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:444114/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:564968/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:421566/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:412681/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:393033/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:380340/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1010342/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:355302/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:352081/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:34325/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:296199/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:868136/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:996205/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:993495/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:989664/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:981536/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:975809/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:958840/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:917196/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:907957/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:889448/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:876677/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:555929/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:858232/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:813035/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:797246/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:761004/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:715587/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:714067/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:691620/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:67982/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:593642/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:378389/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1213760/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:140740/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1365891/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1351080/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1329996/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1329209/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1311077/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:127962/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1234890/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1222224/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:267386/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:115342/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1153/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1130466/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1117812/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:105661/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1055458/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1026624/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1018801/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:167943/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1408006/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:209940/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:227733/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:233913/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:191887/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1438495/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1437564/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:239396/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1436950/62‑1 (MQ=255)gCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1415944/62‑1 (MQ=255) cAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:583009/61‑1 (MQ=255) cAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1331872/61‑1 (MQ=255) cAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCATCGATGATAAAGCGttt < 1:982584/61‑1 (MQ=255) cAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCCCTTCGATGATAAAGCGttt < 1:427628/61‑1 (MQ=255) cacaAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:1034192/54‑1 (MQ=255) caAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:237602/52‑1 (MQ=255) aaaaCGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATGATAAAGCGttt < 1:770746/51‑1 (MQ=255) tCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:40388/60‑1 (MQ=255) cccTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:271097/59‑1 (MQ=255) cccTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:399351/59‑1 (MQ=255) cccTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:526904/59‑1 (MQ=255) cccTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:1242325/59‑1 (MQ=255) cccTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:746754/59‑1 (MQ=255) cccTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:913140/59‑1 (MQ=255) cccTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:1207187/59‑1 (MQ=255) cccTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:1103195/59‑1 (MQ=255) cccTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:207264/59‑1 (MQ=255) cGATAATAAAGCGTTTTGCCGCTGCGCATACCTGTCCGGTATTCTGATAACGTCCGGCt > 1:221695/1‑59 (MQ=255) cGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGc > 1:913756/1‑58 (MQ=255) cGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCt > 1:904871/1‑59 (MQ=255) cGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCt > 1:696405/1‑59 (MQ=255) cGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCt > 1:572757/1‑59 (MQ=255) cGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCt > 1:20816/1‑59 (MQ=255) cGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCt > 1:1024650/1‑59 (MQ=255) aataaAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACg < 1:1177482/48‑1 (MQ=255) | GCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAACGTCCGGCT > W3110S.gb/1615543‑1615647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |