Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,632,595	T→C	T11T (ACA→ACG)	ydfI ←	predicted mannonate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,632,595	0	T	C	100.0%	83.3 / NA	31	T11T (ACA→ACG)	ydfI	predicted mannonate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/0);  total (31/0)

AAATGAACAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTG  >  W3110S.gb/1632548‑1632608                                                |              aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAgcgt             >  1:108970/1‑50 (MQ=38) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCt   >  1:1365606/1‑60 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCt   >  1:759714/1‑60 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:870327/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:869662/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:811839/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:762833/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:899857/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:311310/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:710223/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:678374/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:665371/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:664038/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:517465/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:414102/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:369284/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:982288/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:287203/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:256641/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:163955/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:1460199/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:1454761/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:1418820/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:1416957/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:1308387/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:128674/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:1260666/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:124509/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:1209365/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:1162394/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCTTTTGCTg  >  1:1157263/1‑61 (MQ=255) aaaTGAACAATTCTTGGGGCCAGGTTATTACGATCATAAACAGGGAGCGTCGCGTTTGCTg  >  1:326719/1‑61 (MQ=255)                                                |              AAATGAACAATTCTTGGAGCCAGGTTATTAAGATCATAAACAGGGAGTGTCGCTTTTGCTG  >  W3110S.gb/1632548‑1632608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑