Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,721,565	T→C	intergenic (‑249/‑25)	ydhH ← / → slyB	conserved hypothetical protein/outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,721,565	0	T	C	100.0%	52.2 / NA	20	intergenic (‑249/‑25)	ydhH/slyB	conserved hypothetical protein/outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/20);  total (0/20)

TACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCA  >  W3110S.gb/1721533‑1721589                                 |                         taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:313529/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:996690/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:952675/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:906210/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:865133/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:862511/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:824663/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:782646/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:712084/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:1093641/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:30132/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:281037/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:255442/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:1445784/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:1259713/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:1179800/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:1178102/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:1157671/57‑1 (MQ=255) taCTTTGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTCAACAGGAGATTTTTCa  <  1:47122/57‑1 (MQ=255)    tttGACCATGAGGGATGCTTGCGTGGCGTCTCATGGTGAACAGGAGATTTTTCa  <  1:300104/54‑1 (MQ=255)                                 |                         TACTTTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCA  >  W3110S.gb/1721533‑1721589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑