Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 160,068 C→G Q15H (CAG→CAC)  yadB ← glutamyl‑Q tRNA(Asp) synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb160,0680CG99.0% 268.2 / ‑5.6 96Q15H (CAG→CAC) yadBglutamyl‑Q tRNA(Asp) synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (0/95);  minor base A (0/1);  total (0/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTG  >  W3110S.gb/160017‑160078
                                                   |          
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATTTAGTGTGtgtctg  <  1:530495/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtttctg  <  1:556145/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:404951/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:513708/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:507422/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:502228/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:460442/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:431794/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:428895/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:425675/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:405104/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:284237/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:395653/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:391180/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:374545/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1028420/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:359944/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:342457/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:32299/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:319840/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:291320/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:365013/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:729709/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:95723/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:910386/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:882990/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:832388/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:818813/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:818030/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:784804/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:773052/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:731667/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:526674/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:71817/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:668465/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:599513/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:59727/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:593851/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:589072/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:57998/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:542623/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:257356/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1204760/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1219699/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:119957/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1174027/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1233560/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1285362/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1286231/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1308711/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1313009/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1313816/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1349129/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1359581/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1160691/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:115148/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1131494/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:141433/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:253540/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:233317/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:274128/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:219766/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:202340/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1093381/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1093852/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1453949/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1101188/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1451342/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1446528/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1411139/62‑1 (MQ=255)
agaaCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAATGTGtgtctg  <  1:667700/62‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:768414/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:748743/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1169874/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1104240/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1032049/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:339779/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1217641/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:592804/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:384377/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1264417/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:141673/58‑1 (MQ=255)
 gaaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1399005/58‑1 (MQ=255)
  aaccAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:368272/58‑1 (MQ=255)
     cAAAATGATGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:55276/57‑1 (MQ=255)
     cAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:149086/57‑1 (MQ=255)
              gCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:630522/48‑1 (MQ=255)
              gCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:458400/48‑1 (MQ=255)
                 cGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:694708/45‑1 (MQ=255)
                     ggAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:806190/41‑1 (MQ=255)
                     ggAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1261848/41‑1 (MQ=255)
                     ggAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:648923/41‑1 (MQ=255)
                      gAAGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:880136/40‑1 (MQ=255)
                       aaGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1232931/39‑1 (MQ=255)
                        aGGAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:881926/38‑1 (MQ=255)
                         ggAGAGGGGGCGAAGCGGCCAATATAGTGTGtgtctg  <  1:1366083/37‑1 (MQ=255)
                                                   |          
AGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTG  >  W3110S.gb/160017‑160078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑