Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	197,182	C→T	L213L (CTG→TTG)	yaeL →	zinc metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	197,182	0	C	T	100.0%	95.3 / NA	36	L213L (CTG→TTG)	yaeL	zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/0);  total (36/0)

CTTCGCTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGAA  >  W3110S.gb/197177‑197226      |                                             cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGACCCTGTACTGGaa  >  1:454708/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAAGCTGTACTGGaa  >  1:536776/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTGCTGGaa  >  1:1363734/1‑50 (MQ=38) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:707626/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:511875/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:520560/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:625673/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:626455/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:627353/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:686266/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:70425/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:971812/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:739326/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:75699/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:776928/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:841851/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:892811/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:940174/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:102984/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1420289/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1056231/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1074453/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1082954/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1153945/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1239830/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1363306/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1364943/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:397756/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1429530/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:1431181/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:160757/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:166706/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:193273/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:358634/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGaa  >  1:371735/1‑50 (MQ=255) cTTCGTTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCAGt         >  1:831291/1‑43 (MQ=38)      |                                             CTTCGCTGGGGATTCGTCCTCGTGGGCCGCAAATTGAACCTGTACTGGAA  >  W3110S.gb/197177‑197226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑