Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,922,733 C→A P266H (CCC→CAC)  yebU → predicted methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,922,7330CA100.0% 260.3 / NA 93P266H (CCC→CAC) yebUpredicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (93/0);  total (93/0)

GAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCA  >  W3110S.gb/1922719‑1922779
              |                                              
gagagagACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1053870/4‑61 (MQ=255)
gAAATAGACTTACCACGCCGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:678573/1‑61 (MQ=255)
gAAAGATACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1451084/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTc           >  1:184453/1‑52 (MQ=39)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:440197/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:62037/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:614357/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:609074/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:569281/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:565422/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:559570/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:5583/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:551149/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:476917/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:472339/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:647100/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1000037/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:438286/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:416350/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:403114/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:33358/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:331693/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:329639/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:248809/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:208562/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:964728/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:82134/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:951519/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:941684/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:93016/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:90536/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:899340/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:885617/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:865337/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:863540/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:862382/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:838621/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:955883/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:820311/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:810421/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:796494/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:795930/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:7695/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:766953/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:727254/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:959393/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:669918/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1103857/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:207897/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1311297/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1297894/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1202154/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1194662/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1181149/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1158031/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1144998/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1113032/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1111610/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1109961/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1321655/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1102390/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1085233/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1081853/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1078288/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1064674/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:106215/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1058001/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:105678/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1049556/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1026545/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1440884/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:16240/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:161033/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:160426/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:158232/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:156471/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1463040/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1461883/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1461494/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1449200/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1343838/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1426388/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1422884/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1411276/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1402419/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1377764/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1377638/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1367524/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1364920/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1359008/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGCGca  >  1:275831/1‑61 (MQ=255)
gAAAGAGACTTACCACGACGCAGTAGAGTTTTTACAGCTTGGCGATCTCTTCCCTGGTGc   >  1:602301/1‑60 (MQ=255)
caaaGAGACTTACCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGca  >  1:1034189/2‑61 (MQ=255)
              |                                              
GAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCA  >  W3110S.gb/1922719‑1922779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑