| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,001,926 | C→A | L199L (CTG→CTT) | fliY ← | cystine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,001,926 | 0 | C | A | 80.0% | 110.4 / 25.2 | 60 | L199L (CTG→CTT) | fliY | cystine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/12); new base A (48/0); total (48/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
ACTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCGGG > W3110S.gb/2001882‑2001986 | aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGa > 1:1287106/1‑61 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGa > 1:394546/1‑61 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:422387/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:820239/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:155511/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:165569/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:777400/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:741234/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:270855/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:310221/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:322719/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:696751/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:386175/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:405503/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:796974/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:64787/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:439105/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:64755/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:446512/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:478113/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:628817/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:617074/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:496933/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:50751/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:521837/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:543580/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:552387/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:893967/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:1181544/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:902178/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:1169728/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:1147048/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:1238155/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:1311346/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:928908/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:1104/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:864216/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:1090454/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:1364116/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:930890/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:939313/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:1395193/1‑62 (MQ=255)aCTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCAAGACGGTCAACGAGGAt > 1:946221/1‑62 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:1404275/61‑1 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:461647/61‑1 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:1128830/61‑1 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:543124/61‑1 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:35515/61‑1 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:343772/61‑1 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:1242761/61‑1 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:736150/61‑1 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:1193521/61‑1 (MQ=255) tGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATc < 1:1392554/61‑1 (MQ=255) ccAGACGATCATCGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:49800/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGGGCAGATCCTGATATTTGGTCggg < 1:637015/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggt < 1:906517/62‑2 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:43317/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:846939/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:586135/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:818691/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:81123/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:586559/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:931639/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:589786/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:929113/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:882760/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:67780/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:903540/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:660187/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:53668/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1050532/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1053968/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1079618/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1086932/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1121333/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1212075/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1227434/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1342028/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1345030/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1356871/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1399289/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1418806/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1425420/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1429109/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:203378/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:228275/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:306358/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:329099/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:384745/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:415815/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1020926/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:440304/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:458635/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:484135/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:487758/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:512094/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:548401/62‑1 (MQ=255) ccAGACGATCAACGAGGATCGCATCGATACGCCCTACGCACAGATCCTGATATTTGGTCggg < 1:1110466/62‑1 (MQ=255) cAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:184038/61‑1 (MQ=255) cAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCggg < 1:1033034/61‑1 (MQ=255) | ACTGCCAGCGTATCGTTGGTTTTCTTCACCAGATCCAGCGCCGCCAGACGATCAACGAGGATCGCATCGATACGCCCTACGCGCAGATCCTGATATTTGGTCGGG > W3110S.gb/2001882‑2001986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |