| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,064,317 | A→T | intergenic (‑247/‑80) | nac ← / → asnV | DNA‑binding transcriptional dual regulator/tRNA‑Asn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,064,317 | 0 | A | T | 80.4% | 297.4 / 62.7 | 148 | intergenic (‑247/‑80) | nac/asnV | DNA‑binding transcriptional dual regulator/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base A (13/16); new base T (119/0); total (132/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.21e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 148 total aligned reads displayed.
AAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCA > W3110S.gb/2064285‑2064361 | aaGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:5517/55‑1 (MQ=255)aaGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:286029/55‑1 (MQ=255)aaGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:107614/55‑1 (MQ=255)aaGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:578169/55‑1 (MQ=255)aaGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:452191/55‑1 (MQ=255)aaGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:671281/55‑1 (MQ=255)aaGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:684206/55‑1 (MQ=255)aaGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:1437585/55‑1 (MQ=255)aaGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:73511/55‑1 (MQ=255) aGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGc < 1:113698/54‑1 (MQ=255) gCTCGCCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:247315/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACaaaaaaa > 1:917924/1‑42 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCAttt > 1:1385790/1‑49 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCAttt > 1:1312641/1‑49 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCAttt > 1:718071/1‑49 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCAttt > 1:930155/1‑49 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCAttt > 1:348559/1‑49 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCAttt > 1:721746/1‑49 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:438413/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:318079/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:498078/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:277555/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:455115/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:346887/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:350455/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:442625/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:426871/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:355878/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:368183/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:421215/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:405548/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:384991/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:779341/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:892035/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:887954/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:867045/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:865520/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:847213/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:822468/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:822369/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:807215/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:511909/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:709861/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:687015/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:684623/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:63082/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:588933/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:933207/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:57871/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:567842/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:117820/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1287615/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1276333/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:968254/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:12517/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1249012/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1224397/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1205052/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1201926/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1199630/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1184582/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1292952/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:116313/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:116029/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1129559/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1108732/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1016918/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1095743/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1053533/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1026767/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:102338/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1432421/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:289196/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:283852/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:247800/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:214075/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:19870/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:189013/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1465786/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1454595/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:935461/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1427849/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1409571/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1394162/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:138756/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:132691/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1320582/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:961817/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTc > 1:1306513/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAACAAACAAAAAAATGCATTTCAc > 1:1373223/1‑52 (MQ=255) gCTCACCACTTTGTTGAAATTGTCAGCAAAAAAACAAAAAAATGCAATTc > 1:1090429/1‑50 (MQ=255) gCTCACCACTTTGTTGAAATCGTCAGCAAACAAACAAAAAAATGCAttt > 1:1416920/1‑49 (MQ=39) cacTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCa > 1:971148/1‑61 (MQ=255) cacTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCa > 1:910853/1‑61 (MQ=255) cacTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCa > 1:836075/1‑61 (MQ=255) cacTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCa > 1:304261/1‑61 (MQ=255) cacTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCa > 1:1306997/1‑61 (MQ=255) cacTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCa > 1:1274808/1‑61 (MQ=255) cacTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCa > 1:1105904/1‑61 (MQ=255) cacTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTCTGACATCACCa > 1:586333/1‑61 (MQ=255) | AAGATTGAAAGCTCACCACTTTGTTGAAATTGACAGCAAACAAACAAAAAAATGCATTTCACCCTTTGACATCACCA > W3110S.gb/2064285‑2064361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |