Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,148,022 C→A L874I (CTC→ATC)  yegE → predicted diguanylate cyclase, GGDEF domain signalling protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,148,0220CA100.0% 288.9 / NA 110L874I (CTC→ATC) yegEpredicted diguanylate cyclase, GGDEF domain signalling protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (110/0);  total (110/0)

Only 100 of 110 total aligned reads displayed.

AATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCC  >  W3110S.gb/2148007‑2148053
               |                               
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:432580/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:601561/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:593047/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:580346/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:577035/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:575646/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:553955/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:539439/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:537954/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:535209/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:500561/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:490775/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:448685/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:624983/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:356867/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:334616/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:334232/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:33060/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:298672/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:286521/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:279403/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:267939/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:264080/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1004340/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:22389/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:813771/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:964131/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:954498/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:9474/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:939707/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:909646/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:905581/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:898263/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:894858/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:890597/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:876337/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:831207/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:249189/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:813030/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:797226/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:765968/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:760451/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:759376/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:740208/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:730392/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:692154/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:650559/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:632416/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:631343/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1167314/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1314834/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:201324/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1298676/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1297996/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1265799/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1254889/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1239605/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1230372/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1195932/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1194675/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1173514/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1313775/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1159872/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1155670/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1117668/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1081671/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1074734/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1069352/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1068918/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1039156/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1031862/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:102165/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1009667/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1395022/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:172710/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:172703/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:163258/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1463381/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1456802/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1455737/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1450685/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1414/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1399643/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:13995/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1398044/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1335099/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1392538/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1374033/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1373977/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:136868/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1355361/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1353413/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1338609/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct     >  1:1336394/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggc      >  1:772921/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggc      >  1:1093483/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggc      >  1:1291293/1‑42 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACg        >  1:440082/1‑41 (MQ=25)
aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACGGCTccc  >  1:562710/1‑47 (MQ=25)
aaTCAGTTGATGATGATCGCCCACCGCATCGCTTCGCCACggc      >  1:1262810/1‑42 (MQ=21)
               |                               
AATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCC  >  W3110S.gb/2148007‑2148053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑