Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,148,931 T→C T249T (ACA→ACG)  alkA ← 3‑methyl‑adenine DNA glycosylase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,148,9310TC100.0% 141.3 / NA 51T249T (ACA→ACG) alkA3‑methyl‑adenine DNA glycosylase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/0);  total (51/0)

CGGATTTGCGCCGGTGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGGAA  >  W3110S.gb/2148917‑2148978
              |                                               
cggATTTGTGCCGGCGTCATTCCCGGATATCGCTGTTTAATCAGATAatca             >  1:38952/1‑51 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAAt                       >  1:1010258/1‑41 (MQ=38)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATGATCATCCGGCAGaaa  >  1:149104/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATATTCATCCGGCAGaaa  >  1:1371406/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATcc          >  1:434631/1‑54 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:782561/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:586675/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:594307/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:601864/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:680153/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:692628/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:696184/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:714352/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:734554/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:746997/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:774567/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:547073/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:809808/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:854509/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:867731/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:867784/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:875500/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:886133/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:89661/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:901103/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:927054/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:96437/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:982053/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:249046/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:1032727/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:105567/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:1100855/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:1115023/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:1117852/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:1168679/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:1258433/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:1272877/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:1292910/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:1309977/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:14380/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:580097/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:275023/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:340986/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:344992/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:383021/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:399308/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:460384/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:52834/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:534405/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:549026/1‑62 (MQ=255)
cggATTTGTGCCGGCGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGaaa  >  1:561504/1‑62 (MQ=255)
              |                                               
CGGATTTGCGCCGGTGTCATTCCCGGAAATCGCTGTTTAATCAGATAATCATCCGGCAGGAA  >  W3110S.gb/2148917‑2148978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑