BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	209,571	T→C	G317G (GGT→GGC)	accA →	acetylCoA carboxylase, carboxytransferase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	209,571	0	T	C	100.0%	364.9 / NA	128	G317G (GGT→GGC)	accA	acetylCoA carboxylase, carboxytransferase, alpha subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (128/0); total (128/0)

Only 100 of 141 total aligned reads displayed.

GTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCAC  >  W3110S.gb/209517‑209632

gtTAAGTACTGAATATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:131970/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTAcgctt                                                        >  1:1275024/1‑60 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTAcgcg                                                         >  1:103752/1‑61 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTAcgcg                                                         >  1:595058/1‑61 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTAcgcg                                                         >  1:440638/1‑61 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTAcgcg                                                         >  1:876382/1‑61 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTAcgcg                                                         >  1:1094898/1‑61 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTAcgcg                                                         >  1:144349/1‑61 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTAcgcg                                                         >  1:144300/1‑61 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTAc                                                            >  1:786371/1‑58 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:527140/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:471414/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:497069/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:505715/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:451772/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:543762/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:60241/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:620848/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:456903/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:453074/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:644643/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:444231/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:414980/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:390987/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:381565/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:355626/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:354309/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:346403/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:816696/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:96853/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:958862/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:941815/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:91886/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:899752/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:89897/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:892918/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:878506/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:846582/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:844401/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:636140/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:798687/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:766899/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:722322/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:71685/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:71262/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:710738/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:709935/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:704671/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:678024/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:328805/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1160541/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1285471/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1264237/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1233464/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1223698/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1221995/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1213429/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1191171/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1181798/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:116373/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1161887/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1250139/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1134520/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1129733/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1120244/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1093654/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1085770/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:108395/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1074366/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1072861/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1058143/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1000680/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:142903/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:328600/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:314198/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:296464/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:248728/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:236328/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:181496/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:150525/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1430713/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1425580/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1415901/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1325804/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1412245/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1331463/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1379749/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:1406602/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:138560/1‑62 (MQ=255)

gtTAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCCACGCGt                                                        >  1:754718/1‑62 (MQ=255)

gtTAAGTACTGAAGATTT‑AAAAATCGGCGTTATCAGCGCCTGATGAGCTACGGCTACGCGTa                                                       >  1:1000007/1‑62 (MQ=255)

gatAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCAGATGAgctacggctacg                                                           >  1:1067752/3‑59 (MQ=255)

attAAGTACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGCTACGCGt                                                        >  1:278694/2‑62 (MQ=255)

                                                      ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCa   >  1:1057560/1‑61 (MQ=255)

                                                      ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCAc  >  1:858854/1‑62 (MQ=255)

                                                      ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCAc  >  1:832954/1‑62 (MQ=255)

                                                      ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCAc  >  1:578062/1‑62 (MQ=255)

                                                      ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCAc  >  1:1299544/1‑62 (MQ=255)

                                                      ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCAc  >  1:474191/1‑62 (MQ=255)

                                                      ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCAc  >  1:639089/1‑62 (MQ=255)

GTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCAC  >  W3110S.gb/209517‑209632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`